Glove boxes are used to create a controlled environment, either for isolation of sensitive substances, or to protect users from hazardous materials. The boxes feature built-in gloves for safe handling of the contents.
A vacuum pump is used to remove ambient air from the glove box, which is backfilled with an inert gas, such as argon. This process can be repeated to further lower oxygen concentration. The transfer chambers should also be evacuated to prevent external atmosphere from entering the glove box.