Can columns be reused safely?

Only under tightly controlled conditions. The infographic outlines when reuse is acceptable and what validation steps are required.

How does buffer composition affect cleanup?

Buffer pH and ionic strength directly impact binding and elution efficiency. The infographic includes a quick-reference chart for optimal buffer selection.

Is this guide applicable to automated systems?

Yes. While focused on manual workflows, the principles apply to automated platforms with column-based extraction modules.

What are the signs of poor cleanup?

Low yield, inconsistent purity, and unexpected peaks in chromatograms are common indicators. The infographic shows how to trace these back to specific cleanup steps.

Extraction Column Cleanup

REACH OUT TO US

Column cleanup is often the last step before analysis—but it’s also where contamination, carryover, and sample loss can spike. This infographic breaks down the most common failure points in extraction column workflows and shows how to fix them fast.

What’s inside the infographic

The asset maps out the cleanup process step-by-step, highlighting where errors typically occur and how to prevent them. It covers:

Pre-cleanup prep: How to avoid introducing contaminants during sample loading

Wash steps: Why volume and buffer composition matter more than you think

Elution strategy: How to maximize recovery without compromising purity

Column reuse risks: When reusing columns is acceptable—and when it’s not

Operator technique: How small handling errors lead to big downstream problems

Each section includes visual cues and quick tips for lab teams to apply immediately.

Who should use this

This asset is designed for lab technicians, quality managers, and anyone working with column-based extraction in clinical, pharmaceutical, or research settings. It’s especially useful for teams troubleshooting inconsistent results or contamination spikes.

Download the infographic

FAQs

Skipping or rushing the wash steps. Inadequate washing leaves behind salts and proteins that interfere with downstream analysis.

Extraction Column Cleanup

What’s inside the infographic

Who should use this

FAQs

What’s the most common mistake during column cleanup?

Can columns be reused safely?

How does buffer composition affect cleanup?

Is this guide applicable to automated systems?

What are the signs of poor cleanup?

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